|
KMID : 0390219990100020202
|
|
Journal of Clinical Otolaryngology, Head and Neck Surgery
1999 Volume.10 No. 2 p.202 ~ p.210
|
|
|
Effects of Nitric Oxide and Interleukin-1 Produced by Human Nasal Fibroblast Stimulated by Mitogens and Mycoplasma Antigens
|
|
이한국/Han Kook Lee
김광혁/유태현/이강대/이봉희/이윤우/장명웅/Kwang Hyuk Kim/Tai Hyun Yu/Kang Dae Lee/Bong Hee Lee/Woon Woo Lee/Myung Woong Chang
|
|
|
Abstract
|
|
|
Background and Objectives : Excessive cytokine expression and nitric oxide (NO)
induced by stimuli such as microbial products may be one aspect of the in inflammatory
response associated with bacteremia. We evaluated effect of mitogens and Mycoplasma
antigens on the biological activity of human nasal fibroblast.
Materials and Methods : We have undertaken a study of the kinesics of NO and
interleukin-1 (IL-1) production in human nasal fibroblast culture exposed with
lipopolysaccharide (LPS) of gram negative bacteria, Staphylococcus enterotoxin B (SEB),
or Mycoplasma lysates. This study was designed to evaluate NO and IL-1 in the
fibroblast culture supernatant by assays of nitrite ion concentration and mouse
thymocyte cultivation.
Results : 1) The NO production was increased by 0.01㎍/㎖ of LPS, all doses of SEB
(0.001-1.0㎍/㎖), or all kinds of Mycoplasma lysates(M. pneumoniae, M fermentans, M.
hominis), but decreased by 0.001, 0.1, and 1.0 ㎍/㎖ of LPS. 2) The IL-1 production was
significantly increased by all doses of LFS (0.001-1.0 ㎍/㎖), SEB (0.001-1.0 ㎍/㎖), or
Mycoplasma lysates. 3) In she culture supernatants of the fibroblast exposed to double
stimuli, LPS plus SEB, LPS plus Mycoplasma lysates, the NO concentrations were
higher than in those exposed to LPS alone. 4) The fibroblast responded differently to
double exposure with stimuli in the production of IL-1. The IL-1 concentration increased
or decreased according to doses and kinds of stimuli as compared with those exposed to
LPS alone. Especially, the group exposured with LPS (0.01 ㎍/㎖) plus Mycoplasma
lysates showed highly significant increase of IL-1 as compared with the group exposed
to LPS (0.01 ㎍/㎖) alone.
Conclusion : It is therefore believed that NO and IL-I production in human nasal
fibroblast inclosed mostly by not only LPS, but also SEB. Moreover, its increase by
Mycoplasma lysates was remarkable. Excessive NO and IL-1 production elicited by
double exposure with stimuli in vivo may effect on the inflammatory reactions and
cytokines production.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|